Fromont, J.; Vanderklift, M.A. PLoS ONE. (A) Venn Diagram of OTUs present in two co-occurrence networks inferred for sponge microbiome at inshore (IN) and offshore (OFF) sampling locations. Pyrosequencing reveals highly diverse and species-specific microbial communities in sponges from the Red Sea. Similarity Percentage (SIMPER) analysis was used to determine the OTUs that contribute to the differences in community structure between Eastern Australian samples from locations classified as either inshore (Green, Fantome and Orpheus Islands) or offshore (Davies Reef). As previous sponge symbiosis studies have demonstrated reduced chlorophyll a concentrations under low light conditions (Becerro & Paul, 2004; Erwin & Thacker, 2008), we hypothesized that site characteristics reflecting light levels would drive differences in sponge microbial communities between inshore and offshore environments. GPS coordinates for each site can be found in Table S1. Image detail. eCollection 2019. In contrast, in the present study we saw a 7% increase in cyanobacterial abundance in inshore samples indicating that the microbiome of C. folisacens is more environmentally sensitive than that of X. muta. Wilkinson CR, Trott LA. In Fig. The 16S rRNA genes were amplified by PCR and sequenced as part of the Earth Microbiome Project (EMP) (Gilbert, Jansson & Knight, 2014) on the Illumina platform using the bacterial primers 515F/860R and standard protocols (Caporaso et al., 2012). Cook, S. de C.; Bergquist, P.R. (eds) Systema Porifera - A guide to the classification of sponges. In: Wells, A. You can help Wikipedia by expanding it. Carteriospongia foliascens is a species of sea sponge in the family Thorectidae. Fabricius KE, Logan M, Weeks S, Brodie J. OTUs assigned to Alphaprotobacteria, Bacteroidetes, Gammaproteobacteria and unclassified Proteobacteria contributed most to the ordination, with a spearman rank correlation greater than 0.8 (Fig. Thermal and sedimentation stress are unlikely causes of brown spot syndrome in the coral reef sponge, ianthella basta. OTUs from the SIMPER analysis were visualized using Cytoscape v3.2.1 (www.cytoscape.org) (Shannon et al., 2003). Corresponding sample IDs can be found in Table S1. government site. Wilkinson CR, Cheshire AC. Cytoscape network created from the 30 OTUs (97% similarity) driving the difference between Inshore and Offshore communities identified in SIMPER analysis. ; Maulidina, N. (2019). mSphere. (2009). Bergquist, P.R. of C. foliacens samples from each location. We hypothesize that exclusive taxa are acclimated to these habitats. In addition, there was a significant difference in species diversity (PERMANOVA, Pseudo-F6,65 = 5.87, p = 0.0002) and richness (PERMANOVA, Pseudo-F6,65 = 7.20, p = 0.0001) estimates between locations, with Inverse Simpson values ranging from 9 to 17 and averaged estimated richness (Chao1) ranging from 3,544 to 5,751 OTUs (Table S2). Entries that have been successfully determined and assigned to this entry. In: J. van der Land (ed.) As part of a global sponge microbiome initiative, forming part of the EMP, the microbial communities of 72 C. foliascens individuals collected from multiple locations around tropical Eastern and Western Australia were sequenced (Fig. Notably, community differences in samples from Torres Strait were driven by a higher abundance of two Alphaproteobacteria OTUs (Fig. 5B we show the abundance boxplots for all OTUs affiliated to these two phyla at inshore and offshore locations. The environmental stability of sponge microbial associations varies greatly between species and environmental conditions (Cleary et al., 2013; Crdenas et al., 2014). 2020 Jan 29;5(1):e00481-19. The functional roles of marine sponges. S2). Database (Oxford). Representative sequences were classified based on the SILVA database, using a minimum cutoff of 60%. (B) Abundance boxplot of co-occurring taxa affiliated to the phyla. While samples possessed similar phyla-level diversity, sharing 60% similarity at the phyla-level vs. 20% at the OTU-level (percentages from CLUSTER analysis), the relative abundances of OTUs in some phyla varied between locations (Fig. EMP sample IDs and collection location of samples from this study, including reference to samples used in the inshore/offshore comparison. Carteriospongia foliascens Carteriospongia foliascens (Pallas, 1766) Upload your photos Google image | No photo available for this species. https://link.springer.com/article/10.1007/s00248-014-0419-0. Spongien von Sansibar. Figure 4. NSW was funded by an Australian Research Council Future Fellowship, FT 120100480. Species Carteriospongia foliascens. Moreover, 16S rRNA gene datasets have also been used to infer correlation networks to shed light on possible interactions between microbial species, especially within complex communities. NCBI Taxonomy: a comprehensive update on curation, resources and tools. official website and that any information you provide is encrypted Image content on this page is copyright WA Museum. Species Carteriospongia contorta. Superregnum: Eukaryota Microbial diversity in marine biofilms along a water quality gradient on the Great Barrier Reef. No drawings available for Thorectidae. PCO revealed considerable microbial variation at the OTU level according to host geography, with 24.3% of the total variation in community composition explained in the first two factors (Fig. government site. These OTUs comprised 1,382,146 sequences, with an average of 19,196.47 (7,972.81 1 S.D.) Melbourne, CSIRO. Here we compared the microbiome of an ecologically important sponge species, Carteriospongia foliascens, over a large geographic area and ident We also created a reference transcriptome for the primary symbiont of C. orientalis Gerakladium endoclionum. (urn:lsid:marinespecies.org:taxname:165263), The webpage text is licensed under a Creative Commons Attribution 4.0 License, (urn:lsid:marinespecies.org:taxname:165263). 1028-1050. Text content on this page is licensed under a Creative Commons Attribution 4.0 International License. Species Carteriospongia delicata. (2 volumes) Kluwer Academic/ Plenum Publishers: New York, 1708 + xvliii. Similarly, in the few studies that have undertaken in situ analysis of microbial communities in sponges under varying irradiance or pCO2 levels, different species hosted either stable or variable microbial communities in the face of the altered environmental conditions (Morrow et al., 2014; Freeman et al., 2015). Foto: Rotes Meer, gyptenUploaded by spacediver. -, Becerro MA, Paul VJ. Here, we established reproductive parameters including mode of sexuality and development, seasonality, sex ratios, gametogenesis, reproductive output, and size at sexual maturity for the common phototrophic intertidal sponge, Carteriospongia foliascens, in the central GBR over two reproductive cycles. Wenn Sie diese Seite weiter verwenden, erklren Sie sich damit einverstanden. (ed.) Steindler L, Huchon D, Avni A, Ilan M. 16S rRNA phylogeny of sponge-associated cyanobacteria. Breakdown of the number of OTUs identified (97% similarity) per phyla, class for Proteobacteria. List of OTUs contributing most to the discrimination, Spearman Rank correlation <0.8. Clear groupings by sample location are also evident when looking at the most abundant OTUs, and consistent with the total dataset, Fantome and Orpheus Island samples share the greatest similarity (80%). Phyllospongia) flabellifera, and other unidentified Phyllospongia spp. (2008). Records of the Western Australian Museum. Supplement 77: 89-103. Lendenfeld, R. von. Hoegh-Guldberg (eds). Bell JJ. Spatial patterns in host-associated and free-living bacterial communities across six temperate estuaries. Pp 170-186. Cite this page In fact, a significant correlation between geographic distance and community composition was identified through a comparison of the two matrices (RELATE; Rho = 0.771, P = 0.015). Although different sequencing platforms preclude a direct comparison between studies, it is interesting to note that C. foliascens from the Red Sea hosts a similar phyla level diversity but much lower OTU level diversity than the GBR C. foliascens (Gao et al., 2014a). OTU classification denoted within the nodes. Given that light is one of the most important factors influencing phototrophic sponge distributions (Wilkinson & Trott, 1985), sponges like C. foliascens are more commonly found between 02 m on turbid inshore reefs (Abdul Wahab et al., 2014a) compared to 1030 m in less turbid environments on mid-shelf reefs (Wilkinson & Evans, 1989). Federal government websites often end in .gov or .mil. 2013;19:26132624. Symbiotic adaptation drives genome streamlining of the cyanobacterial sponge symbiont. Can form a cup with internal spirals at maturity. Samples were clustered by location using the Cluster analysis in PRIMER/PERMANOVA (top). 2013; Luter et al. Catchments and corals: terrestrial runoff to the Great Barrier Reef. Therefore, the highest mean relative abundances of this group in sponges collected from inshore locations, where nutrients are likely abundant, are consistent with one of the proposed physiologies for Bacteroidetes. These species provide habitats for a wide range of reef taxa and contribute to primary production via their productive symbioses with cyanobacteria (Wilkinson, 1983; Webster et al., 2013). For instance, the giant barrel sponge Xestospongia muta maintained a stable microbial community between 10100 m (Olson & Gao, 2013), indicating a remarkable stability despite the reduction in light required for phototrophy. China Science Press. 1267 pp. The horizontal line represents where the 30 th OTU is placed, with the top 30 OTUs representing 68% of the accumulated proportion of abundance. Microbiome. These statistical co-occurrence patterns can be used as a filter for functionality (in the case of ecological interactions) and offer insights into canalizing environmental conditions (e.g., environmental gradients) that explain the co-presence of two taxa, which is of particular interest for mechanistically-driven studies (Berry & Widder, 2014; Widder et al., 2014). Species Carteriospongia cordifolia. ISBN 978-1-4615-0747-5 (eBook electronic version). The Great Barrier Reef: Biology, Environment and management. A significant co-occurrence event (edge) was placed if the SparCC correlation coefficient |0.6| and p 0.03. Muhammad Abdul Wahab conceived and designed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper. PMC An official website of the United States government. Rank abundance plot of C. foliacens OTUs. (eds) Systema Porifera - A guide to the classification of sponges. ; Ayling, A.M.; Wilkinson, C.R. Webster NS, Luter HM, Soo RM, Bott ES, Simister RL, Abdo D, Whalan S. Same, same but different: symbiotic bacterial associations in GBR sponges. PRIMER/PERMANOVAs Mantel-type test, RELATE, was used to compare similarity matrices and hierarchal clustering of sample locations was achieved using CLUSTER analysis. Specifically, the following OTUs were the primary drivers of dissimilarity between inshore and offshore locations (accounting for 2.65% total dissimilarity): OTU12154 (Gammaproteobacteria), which was completely absent from inshore samples, OTU669 (Alphaproteobacteria), which was more abundant in offshore samples, and OTU5304 (Cyanobacteria) and OTU2505 (Bacteroidetes), which were both more abundant in inshore samples (Fig. Nicole S. Webster conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper. Hooper, J.N.A. You have to own the copyright to the photo. Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fierer N, Owens SM, Betley J, Fraser L, Bauer M, Gormley N, Gilbert JA, Smith G, Knight R. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. 2022 Jun 29;10(1):99. doi: 10.1186/s40168-022-01286-z. Genus: Carteriospongia This service is powered by LifeWatch Belgium, https://www.biodiversitylibrary.org/page/6019361, https://biodiversity.org.au/afd/taxa/PORIFERA/checklist, To Biodiversity Heritage Library (1 publication), To GenBank (3910 nucleotides; 0 proteins), To Sponge Barcoding Database (Carteriospongia foliascens), To Yale Peabody Museum of Natural History (YPM IZ 005031.PR). CSIRO Publishing: Melbourne. The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers): Permit for the study: Great Barrier Reef Marine Park Authority Permit #G12/35236.1. Unique sequences were aligned against a trimmed SILVA database (v102, trimmed to the V4 region) and chimeric sequences identified by UCHIME (Edgar et al., 2011) were removed. Reiswig HM. Singletons, i.e., OTUs formed by one sequence across all samples, were removed. A total of 9,401 OTUs, spanning 15 bacterial phyla, were identified across all individuals at 97% sequence similarity. Here, individuals of the sponge Carteriospongia foliascens under abnormal status were collected from the Rabigh Bay along the Red Sea coast. Characterised microscopically by vermiform fibres. CSIRO Publishing: Melbourne. Phylum: Porifera and transmitted securely. Family Thorectidae Bergquist, 1978. You can help Wikipedia by expanding it. Kindt R, Coe R. Tree diversity analysis. From the union of OTUs, we extracted the abundance of Cyanobacteria and Bacteroidetes and calculated their ratios in both environments. Luter HM, Whalan S, Webster NS. Carteriospongia foliascens is a widespread and abundant sponge on the GBR that hosts dense populations of cyanobacteria that contribute to primary production and nutrient cycling (Wilkinson 1983; Webster et al. 4 and Table S4). -, Bell JJ, Davy SK, Jones T, Taylor MW, Webster NS. Scalarane-Based Sesterterpenoid RCE-Protease Inhibitors Isolated from the Indonesian Marine Sponge Carteriospongia foliascens. Pp. (1897). The C. foliascens bacterial community was primarily comprised of Gammaproteobacteria (34.2% 3.4%) and Cyanobacteria (32.2% 3.5%), with lower abundances of Alphaproteobacteria, Bacteroidetes, unidentified Proteobacteria, Actinobacteria, Acidobacteria and Deltaproteobacteria. Identification of Sponge-Associated Bacteria From the Coast of Kuwait and Their Potential Biotechnological Applications. -, Abdul Wahab MA, Fromont J, Whalan S, Webster N, Andreakis N. Combining morphometrics with molecular taxonomy: how different are similar foliose keratose sponges from the Australian tropics? As expected, we found a significantly higher prevalence of Cyanobacteria over Bacteroidetes in oligotrophic, light-rich offshore locations (CIBI = 0.25, COBO = 1.48, ANOVA, p < 0.0001) supporting our hypothesis that the community structure of the specialized sponge microbiome is biased by environmental conditions. Liu, J.Y. (1994). Received 2015 Aug 6; Accepted 2015 Nov 3. Species Carteriospongia fissurella. Schmitt S, Tsai P, Bell J, Fromont J, Ilan M, Lindquist N, Perez T, Rodrigo A, Schupp PJ, Vacelet J, Webster N, Hentschel U, Taylor MW. Marine Ecology Progress Series. Both NWs show comparable size (NI = 93, EI = 98; NO = 133, EO = 396 where N = Nodes and E = Edges), but when comparing their fragmentation (e.g., the relative fraction of disconnected compartments within a NW) we find that the inshore NW is more fragmented (f = 0.61) than the offshore counterpart (f = 0.52). Notably, all Cyanobacteria OTUs share high sequence similarity with clones (Otu5304 [98%] and Otu2788 [97%]: {"type":"entrez-nucleotide","attrs":{"text":"KP792324","term_id":"821184232"}}KP792324 98 & Otu115 [98%]: {"type":"entrez-nucleotide","attrs":{"text":"KJ008094","term_id":"597437704"}}KJ008094) from the sponge-specific clade Synechococcus spongiarum (Steindler et al., 2005; Gao et al., 2014b). Keywords: doi: 10.3354/meps280115. doi: 10.1371/journal.pone.0098181. (1988). Pp. A significant correlation was identified between distance matrices from the total dataset and the 30 most abundant OTUs (RELATE; Rho = 0.939, P = 0.001), further supporting the consistency between the two datasets. ; Wiedenmayer, F. (1994). For example, to the right of the ordination plot there was a general grouping of GBR communities (excluding Torres St). Bergquist, P.R., Ayling, A. M., & Wilkinson, C. R. (1988). Cladus: Holozoa 3A). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. After 24 h exposure to . The number of specialist (51, 91) and generalist taxa (42) are shown. Abdul Wahab MA, de Nys R, Webster N, Whalan S. PLoS One. Porifera (sponges) of Mermaid, Scott and Seringapatam Reefs, north Western Australia. Body flexible, fibrous, tears easily longitudinal. Bell JJ, Davy SK, Jones T, Taylor MW, Webster NS. For faster navigation, this Iframe is preloading the Wikiwand page for Carteriospongia foliascens . Sponges of the Seychelles. Classification / Names Common names | Synonyms | CoL | ITIS | WoRMS Demospongiae | Dictyoceratida | Thorectidae